| Acronym | Meaning |
| PLO | Pacific Launch Operation |
| PLO | Partnership Liaison Office |
| PLO | Patrol Leader's Order (US DoD) |
| PLO | Pay Liaison Officer |
| PLO | Permanent Latrine Officer :-) |
| PLO | Permanent Latrine Orderly :-) |
| PLO | Physical Layer Operations |
| PLO | Platoon Leader's Order (US Navy) |
| PLO | Pre-Light Off |
| PLO | Prison Law Office (California) |
| PLO | Public Land Order |
| PLO | Publicity Liaison Officer |