| Acronym | Meaning |
| BOT | Air Botswana (ICAO code) |
| BOT | Bank of Tanzania (est. 1965) |
| BOT | Basic Officer Training |
| BOT | Basic Orientation Training |
| BOT | Bearings-Only Tracking |
| BOT | Beginning Of Tour |
| BOT | Beginning of Track |
| BOT | Blanket Open Test Assembly |
| BOT | Board of Occupational Therapy (various locations) |
| BOT | Bomb on Target (US DoD) |
| BOT | Boom Operator Trainer (US DoD) |
| BOT | Botswana |
| BOT | Bottom of Tape |
| BOT | British Overseas Territory |
| BOT | Broad Operational Task |
| BOT | Build-Operate-Transfer (project outsourcing; information technology delivery model) |
| BOT | Bureau of Treasury (Philippines) |
| BOT | Burst On Target |