| Acronym | Meaning |
| DOI | Daily Operating Instruction |
| DOI | Date of Incorporation |
| DOI | Date of Injury |
| DOI | Date of Introduction |
| DOI | Date of Issuance |
| DOI | Daughters of Iraq (female security program; est. 2008) |
| DOI | Declaration of Independence |
| DOI | Declaration of Intent (legal) |
| DOI | Defense Operating Instruction(s) |
| DOI | Department of Infrastructure (Australia) |
| DOI | Department of Institutions (Colorado) |
| DOI | Department of Intelligence |
| DOI | Department of Investigation |
| DOI | Department of the Interior (US) |
| DOI | Descent Orbit Insertion |
| DOI | Description, Operation & Installation |
| DOI | Detachment Operating Instruction |
| DOI | Diffusion of Innovations |
| DOI | Digital Orthorectified Imagery |
| DOI | Director of Intelligence |
| DOI | Directorate of Instruction |
| DOI | Division of Instruction (various locations) |
| DOI | Division of Insurance |
| DOI | DSSCS (Defense Special Security Communications System) Operating Instructions (US DoD) |