| Acronym | Meaning |
| MAST | Maintenance Standardisation Teams (Europe) |
| MAST | Maintenance Standardization Team |
| MAST | Major Airframe Static Test |
| MAST | Mandatory Alcohol Server Training (Washington state Liquor Control Board) |
| MAST | Mandatory and Statutory Training (National Health Service; UK) |
| MAST | Marine Academy of Science and Technology |
| MAST | Maritime Analysis Support Team (US Coast Guard) |
| MAST | Maritime Security and Strategic Toolkit (US FEMA) |
| MAST | Mega Ampere Spherical Tokamak (UK; nuclear fusion experiment) |
| MAST | Membrane Applied Science and Technology Center (University of Colorado at Boulder and University of Cincinnati) |
| MAST | MICAP Automated Status Tracking |
| MAST | Microcomputer Authoring System Trainer |
| MAST | Midwestern Alliance of Sovereign Tribes |
| MAST | Military Anti-Shock Trousers |
| MAST | Military Assistance to Safety & Traffic (US Army MEDEVAC civilian assistance) |
| MAST | MILSTAR Advanced Satellite Terminal |
| MAST | Mission Avionics Systems Trainer |
| MAST | Mitigation Advisors’ Strategic Tracker (US FEMA) |
| MAST | Mobile Acoustic Spatial Target |
| MAST | Mobile Army Surgical Team |
| MAST | Mobile Ashore Support Terminal |
| MAST | Modeling And Simulation Team |
| MAST | Multi-Application Sonar Trainer |
| MAST | Multi-Int Analysis Software Toolset |