| Acronym | Meaning |
| DEO | Deduction from Earnings Order (UK) |
| DEO | Defence Estate Officer (India) |
| DEO | Defense Employment Option |
| DEO | Department of Economic Opportunity |
| DEO | Deputy Education Officer |
| DEO | Deputy Ethics Official (various government agencies) |
| DEO | Deputy Executive Officer |
| DEO | Detention Enforcement Officer (Department of Homeland Security) |
| DEO | Digital End Office |
| DEO | Digital Entrepreneurship Office (Anambra, Nigeria) |
| DEO | Direct Entry Officer |
| DEO | District Education Office |
| DEO | District Engineer Office |
| DEO | Diversification de l'Économie de l'Ouest Canada (Western Economic Diversification Canada) |
| DEO | Division of Enterprise Operations (Wisconsin) |