| Acronym | Meaning |
| SPO | Safety Project Office |
| SPO | Section Panel Operator |
| SPO | Security and Plans Office/Officer |
| SPO | Security and Plans Operations |
| SPO | Security Police Officer |
| SPO | Sektorowe Programy Operacyjne (Polish: Sectoral Operational Program) |
| SPO | Senate Press Office (Georgia) |
| SPO | Senior Patrol Officer (US government) |
| SPO | Senior Programme Officer |
| SPO | Shore Patrol Officer (US Navy) |
| SPO | Shuttle Projects Office (US NASA) |
| SPO | Signal Property Office |
| SPO | Single-Pilot Operation (aviation; various organizations) |
| SPO | Social Policy Obligations |
| SPO | Space Program Office |
| SPO | Spacelab Projects Office (NASA) |
| SPO | Special Police Officer |
| SPO | Special Program Office/Officer |
| SPO | Special Project Officer |
| SPO | Special Projects Office |
| SpO | Specialist, Inspector of Naval Material (US Navy) |
| SPO | Sponsored Projects Officer |
| SPO | State Procurement Office (various locations) |
| SPO | State Purchasing Officer (various locations) |
| SPO | Statutory Publications Office (UK) |
| SPO | Stoker Petty Officer (UK) |
| SPO | Strategic Performance Objectives |
| SPO | Strategic Plan Objectives (various locations) |
| SPO | Strategic Planning Office (various locations) |
| SPO | Student Police Officer |
| SPO | Summit Planning Organization |
| SPO | Supervisory Police Officer (Pakistan) |
| SPO | Support Operations Officer |
| SPO | System(s) Program Office |
| SPO | System(s) Project Office |